DNA-Labeling Buffer (10×)
| Reagent | Quantity (for 1 mL) | Final concentration |
|---|---|---|
| NaCl (5 m) | 100 μL | 500 mm |
| MgCl2 (1 m) | 100 μL | 100 mm |
| Tris (1 m, pH 7.5) | 100 μL | 100 mm |
| Dithiothreitol (DTT) (1 m) | 10 μL | 10 mm |
| H2O | 690 μL | |
| Store for up to 1 yr at −20°C. | ||
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