Modified Balanced Salt Solution (MBSS)
| Reagent | Final concentration (mm) |
|---|---|
| NaCl | 125 |
| KCl | 2.5 |
| CaCl2 | 2 |
| MgSO4 | 1.3 |
| NaH2PO4 | 1.25 |
| NaHCO3 | 25 |
| Glucose | 10 |
| Oxygenate the MBSS by bubbling it with 95% O2. | |
- © 2013 Cold Spring Harbor Laboratory Press
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doi:10.1101/pdb.rec080200 Cold Spring Harb Protoc 2013. 2013: pdb.rec080200- © 2013 Cold Spring Harbor Laboratory Press
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