Collagenase Solution for Organoids
| Reagent | Final concentration |
|---|---|
| DMEM/F12 (GIBCO) | 1× |
| Fetal bovine serum (FBS; heat-inactivated) | 5% (v/v) |
| Gentamicin | 50 µg/mL |
| Insulin (Sigma-Aldrich) | 5 µg/mL |
| Trypsin (GIBCO) | 2 mg/mL |
| Collagenase A (type IV from Clostridium histolyticum) (Sigma-Aldrich) | 2 mg/mL |
- © 2013 Cold Spring Harbor Laboratory Press
