Recipe

Organoid Medium

Reagent Final concentration
DMEM/F12 (GIBCO) 1×
Penicillin/streptomycin solution (100×) 1×
ITS (insulin/transferrin/sodium selenite; 100×) (Sigma-Aldrich) 1×
This is simple organoid medium (without growth factor). To prepare branching organoid medium, add fibroblast growth factor 2 (FGF2) to a concentration of 1–10 nm (typically 2.5 nm).
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  1. doi:10.1101/pdb.rec073601 Cold Spring Harb Protoc 2013: pdb.rec073601- © 2013 Cold Spring Harbor Laboratory Press
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