Recipe

Lysis Buffer for Plasmid Preparation

Reagent Quantity (for 500 mL) Final concentration
Tris (1 m, pH 8.0) 12.5 mL 25 mm
EDTA (0.5 m) 10 mL 10 mm
Glucose 4.5 g
H2O to 500 mL
Store at 4°C.
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  1. doi:10.1101/pdb.rec073619 Cold Spring Harb Protoc 2013: pdb.rec073619- © 2013 Cold Spring Harbor Laboratory Press
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