T7 Transcription Buffer (10×)
| Reagent | Quantity (for 1 mL) | Final concentration |
|---|---|---|
| Tris–HCl (1 m, pH 8.0) | 400 µL | 400 mm |
| MgCl2 (1 m) | 200 µL | 200 mm |
| DTT (dithiothreitol; 1 m) | 50 µL | 50 mm |
| H2O | 350 µL | |
| Store for 6 mo at –20°C. | ||
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