Annealing Buffer (6×)
| Reagent | Quantity (for 1 mL) |
Final concentration (6×) |
|---|---|---|
| Tris–HCl (1 m, pH 7.4) | 60 μL | 60 mm |
| KCl (2 m) | 150 μL | 300 mm |
| H2O | 0.79 mL | |
| Store indefinitely at –20°C. | ||
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