Recipe

Danilchik's for Amy (DFA) Medium

Reagent Final
concentration
NaCl   53 mm
Na2CO3   5 mm
Potassium gluconate   4.5 mm
Sodium gluconate  32 mm
CaCl2   1 mm
MgSO4   1 mm
Bovine serum albumin    1 g/L
Adjust pH to 8.3 with 1 m bicine before adding bovine serum albumin. (This
recipe is adapted from Sater et al. 1993.)

REFERENCES

    1. Sater AK,
    2. Steinhardt RA,
    3. Keller R
    Sater AK, Steinhardt RA, Keller R. 1993. Induction of neuronal differentiation by planar signals in Xenopus embryos. Dev Dyn 197: 268–280.
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  1. doi:10.1101/pdb.rec074401 Cold Spring Harb Protoc 2013: pdb.rec074401- © 2013 Cold Spring Harbor Laboratory Press
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