T1 Sequencing Buffer
| Reagent | Quantity (for 1 mL) |
Final concentration |
|---|---|---|
| Sodium acetate (3 m, pH 5.3) | 6.6 μL | 20 mm |
| Urea (molecular biology grade) | 0.42 g | 7 m |
| EDTA (0.5 m, pH 8.0) | 2 μL | 1 mm |
| H2O | adjust to 1 mL | |
| Store for 6 mo at room temperature. | ||
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