Recipe

Loading Dye

Reagent Quantity
(for 10 mL) 		Final
concentration
Urea (ultrapure)  5.4 g      9 m
Xylene cyanol/bromophenol blue (1%, w/v) (optional) 500 μL 0.05% (w/v)
Add enough 10× TBE electrophoresis buffer (diluted to 1×) to reach the 10-mL
mark in a 15-mL conical tube containing urea. Heat at 50°C until dissolved.
Store at room temperature. Make sure urea is in solution before using.
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  1. doi:10.1101/pdb.rec074609 Cold Spring Harb Protoc 2013: pdb.rec074609- © 2013 Cold Spring Harbor Laboratory Press
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