Reaction Buffer A (Native)
| Reagent | Quantity (for 100 mL) |
Final concentration |
|---|---|---|
| HEPES/KOH (1 m, pH 7.5) | 1 mL | 10 mm |
| KCl (2 m) | 5 mL | 100 mm |
| MgCl2 (1 m) | 0.5 mL | 5 mm |
| H2O | 93.5 mL | |
| Store indefinitely at 4°C. Adjust MgCl2 concentration between 5 and 25 mm for individual substrates. |
||
- © 2013 Cold Spring Harbor Laboratory Press
