Recipe

IP-Astrocyte Base Medium

Reagent Final concentration
Neurobasal Medium (Gibco/Life
Technologies 21103) 		50%
DMEM (Gibco/Life Technologies 11960-044) 50%
Penicillin-streptomycin (Gibco/Life
Technologies 15140-122) 		100 U/mL (penicillin) 100
µg/mL (streptomycin)
Sodium pyruvate (100 mm; Gibco/Life
Technologies 11360-070) 		1 mm
l-glutamine (200 mm; Gibco/Life
Technologies 25030-081) 		292 µg/mL
SATO supplement, neurobasal-based (100×) 1×
NAC stock (5 mg/mL) 5 µg/mL
Filter-sterilize through a 0.22-μm filter. Store at 4°C in the dark.
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  1. doi:10.1101/pdb.rec075671 Cold Spring Harb Protoc 2013: pdb.rec075671- © 2013 Cold Spring Harbor Laboratory Press
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