MD-Astrocyte Growth Medium

Recipe

Reagent	Volume	Final concentration
DMEM (Gibco/Life Technologies 11960-044)	430 mL	86%
FCS (100%; heat-inactivated for 30 min at 55°C; Gibco/Life Technologies 10437-028)	50 mL	10%
Penicillin-streptomycin (100×; Gibco/Life Technologies 15140-122)	5 mL	1×
l-glutamine (200 mm; Gibco/Life Technologies 25030-081)	5 mL	2 mm
Sodium pyruvate (100 mm; Gibco/Life Technologies 11360-070)	5 mL	1 mm
Insulin stock (0.5 mg/mL)	5 mL	5 µg/mL
NAC stock (5 mg/mL)	0.5 mL	5 µg/mL
Hydrocortisone stock (10 mm in DMSO; Sigma-Aldrich H0888)	0.5 mL	10 µm

Filter-sterilize through a 0.22-μm filter. The final volume is 500 mL. Store at 4°C in the dark.