Recipe

HPLC Buffer B

Dilute HPLC buffer A 1:1 with methanol (HPLC grade; e.g., LiChrosolv from Millipore). (Do not top up the buffer with methanol because volume depression occurs when water and methanol are mixed.) Sonicate HPLC buffer B for 5 min to release air bubbles. The buffer can be stored for 2–3 d at room temperature.

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  1. doi:10.1101/pdb.rec074658 Cold Spring Harb Protoc 2013: pdb.rec074658- © 2013 Cold Spring Harbor Laboratory Press
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