Buffer B (Low Salt)
| Reagent | Quantity (for 100 mL) | Final concentration |
|---|---|---|
| Tris-HCl (1 m, pH 7.9) | 2 mL | 20 mm |
| Glycerol (50%, ultrapure) | 50 mL | 25% (v/v) |
| MgCl2 (1 m) | 0.15 mL | 1.5 mm |
| EDTA (0.5 m, pH 8.0) | 0.04 mL | 0.2 mm |
| KCl (3 m) | 0.66 mL | 20 mm |
| H2O | to 100 mL | |
| Store at 4°C. | ||
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