Recipe

S100 Buffer (10×)

Reagent Quantity (for 100 mL) Final concentration
Tris–HCl (1 m, pH 7.9)   30 mL  0.3 m
KCl (3 m)  46.6 mL  1.4 m
MgCl2 (1 m)    3 mL 0.03 m
H2O to 100 mL
Store at 4°C.
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  1. doi:10.1101/pdb.rec075788 Cold Spring Harb Protoc 2013: pdb.rec075788- © 2013 Cold Spring Harbor Laboratory Press
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