Recipe

EBSS Stock (10×)

Reagent Quantity (for 250 mL) Final concentration (10×)
NaCl   17 g  1.16 m
KCl   1 g  54 mm
NaH2PO4•H2O  0.35 g  10 mm
Glucose  2.5 g   1%
Phenol red (0.5%) 2.5 mL 0.005%
Bring to 250 mL with ddH2O and filter to sterilize.

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  1. doi:10.1101/pdb.rec075390 Cold Spring Harb Protoc 2013: pdb.rec075390- © 2013 Cold Spring Harbor Laboratory Press
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