Recipe

Krebs-Ringer Modified Buffer (KRB)

Reagent Final concentration
NaCl 135 mm
KCl  5 mm
MgSO4  1 mm
K2HPO4  0.4 mm
Glucose  5.5 mm
HEPES  20 mm
Adjust the pH to 7.4. Most experiments are performed in buffer supplemented with 1 mm CaCl2.
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  1. doi:10.1101/pdb.rec076406 Cold Spring Harb Protoc 2014: pdb.rec076406- © 2014 Cold Spring Harbor Laboratory Press
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