Intracellular Buffer
| Reagent | Final concentration |
|---|---|
| KCl | 130 mm |
| NaCl | 100 mm |
| K2PO4 | 20 mm |
| HEPES | 200 mm |
| Succinic acid | 50 mm |
| Malic acid | 10 mm |
| Pyruvate | 10 mm |
| MgCl2 | 10 mm |
| Adjust the pH to 7.0 with KOH. | |
- © 2014 Cold Spring Harbor Laboratory Press
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doi:10.1101/pdb.rec076414 Cold Spring Harb Protoc 2014. 2014: pdb.rec076414- © 2014 Cold Spring Harbor Laboratory Press
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