Recipe

bFGF (50 µg/mL)

Add 1 mL of 5 mm Tris (pH 7.6)/0.1% BSA to a 50-μg vial of bFGF powder (recombinant human FGF-basic; PeproTech 100-18B). Aliquot the solution. Store the aliquots at −20°C to −30°C. Dilute 1000× for use in cell medium.

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  1. doi:10.1101/pdb.rec077289 Cold Spring Harb Protoc 2014: pdb.rec077289- © 2014 Cold Spring Harbor Laboratory Press
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