Hormone Mix (200×)
| Reagents | Amount to add | Final concentration |
|---|---|---|
| Apotransferrin (Sigma-Aldrich T1147; 4 mg/mL in DMEM) | 5 mL | 1 mg/mL |
| Putrescine (Sigma-Aldrich P5780; 12.8 mg/mL in DMEM) | 5 mL | 20 mm |
| Progesterone (Sigma-Aldrich P8783; 25 µg/µL in ethanol) | 10 µL | 4 µm |
| Sodium selenite (Sigma-Aldrich S5261; 0.4 mg/mL in DMEM) | 51.9 µL | 6 µm |
| Combine the ingredients in an additional 10 mL of DMEM. Mix well and filter through a 0.22-µm filter. Make 200-µL aliquots and store at −20°C. | ||
- © 2014 Cold Spring Harbor Laboratory Press
