ND-SATO Base Medium

Recipe

Reagent	Amount to add (for 40 mL)
Neurobasal solution (Gibco 21103-049)	19.5 mL
DMEM (Gibco 11960-044)	19.5 mL
Penicillin/streptomycin (100×) (Gibco 15140-122)	400 µL
l-Glutamine (200 mm) (Gibco 25030-081)	400 µL
SATO (100×)	400 µL
Sodium pyruvate (100 mm) (Gibco 11360-070)	400 µL
Insulin stock (0.5 mg/mL)	400 µL
T3 stock (4 µg/mL)	400 µL
NAC stock (5 mg/mL)	40 µL
Trace Elements B (1000×) (Cellgro)	40 µL
Biotin stock (5000×)	8 µL
B-27 (Invitrogen) or N21-MAX (R&D Systems)	800 µL
Filter the solution through a 0.22-µm filter to sterilize and store at 4°C for up to 1 wk.