Recipe

ND-Growth Medium (ND-G)

Reagent Amount to add
ND-SATO base medium 8 mL
BDNF (Peprotech 450-02; 50 µg/mL in D-PBS containing 0.2% BSA)  8 µL
CNTF (10 µg/mL)  8 µL
Forskolin stock (4.2 mg/mL)  8 µL
Equilibrate in an incubator to 37°C/10% CO2.
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  1. doi:10.1101/pdb.rec077107 Cold Spring Harb Protoc 2014: pdb.rec077107- © 2014 Cold Spring Harbor Laboratory Press
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