MyM Base Medium

Recipe

Reagent	Amount to add (for 40 mL)
DMEM-High Glucose medium (Gibco 11960-044)	39 mL
Insulin stock (0.5 mg/mL)	400 µL
Sodium pyruvate (100 mm) (Gibco 11360-070)	400 µL
l-Glutamine (200 mm) (Gibco 25030-081)	400 µL
Hormone mix (200×)	200 µL
T3 stock (4 µg/mL)	200 µL
Hydrocortisone (50 µm) (Sigma-Aldrich H6909)	40 µL
Trace Elements B (1000×) (Cellgro)	40 µL
Biotin stock (5000×)	8 µL
Vitamin B12 (Sigma-Aldrich V6629; 1.36 mg/mL in D-PBS)	8 µL
B-27 (Invitrogen) or N21-MAX (R&D Systems)	15 µL
Ceruloplasmin (1 mg/mL) (Calbiochem 239799)	4 µL
Filter the solution through a 0.22-µm filter to sterilize and store at 4°C for up to 1 wk.