| Dulbecco’s Modified Eagle Medium (DMEM) (high glucose) (Life Technologies 11965-126) |
450 mL |
|
| Fetal bovine serum (FBS) (Life Technologies 16000-044) |
50 mL |
10% |
| Penicillin/streptomycin (5000 U/mL; Life Technologies 15070-063) |
5 mL |
1% |
| l-Glutamine (200 mm; Life Technologies 25030-081)
|
5 mL |
2 mm |
| HEPES (1 m; Life Technologies 15630-080)
|
5 mL |
10 mm |
| MEM Non-Essential Amino Acids (NEAA) (100×; Life Technologies 11140-050) |
5 mL |
0.1 mm |
| Sodium pyruvate (100 mm; Life Technologies 11360-070)
|
5 mL |
1 mm |
| Sterilize with a 0.2-µm filter. Store at 4°C. |
