TNB Blocking Solution
| Reagent | Amount | Final Concentration |
|---|---|---|
| Tris–HCl (1 m, pH 7.5) | 50 mL | 100 mm |
| NaCl (5 m) | 15 mL | 150 mm |
| Bovine serum albumin (BSA) | 1.8 g | 0.36% (w/v) |
| H2O | to 495 mL | – |
| Heat solution for ∼3 h to 60°C under continuous stirring. Store 49.5-mL aliquots at −20°C. On the day of use, thaw one aliquot, filter it with a 0.22-µm syringe filter, and add fresh skim milk to 1%. | ||
- © 2014 Cold Spring Harbor Laboratory Press
