Ringer’s Solution for Measuring Ca2+ Fluxes across the Mitochondrial Inner Membrane
| Reagent | Final concentration |
|---|---|
| CaCl2 | 1.8 mm |
| Glucose | 15 mm |
| HEPES–HCl (pH 7.4) | 20 mm |
| KCl | 5 mm |
| MgCl2 | 0.8 mm |
| NaCl | 120 mm |
| NaOH | 10 mm |
| Store at 4°C for up to 1 mo. | |
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