Ca2+ Loading Solution for Measuring Ca2+ Fluxes across the Mitochondrial Inner Membrane
| Reagent | Final concentration |
|---|---|
| CaCl2 | 60 µm |
| Cyclosporin A | 1 µm |
| EGTA | 0.4 mm |
| HEPES–KOH (pH 7.4) | 10 mm |
| KH2PO4 | 2.5 mm |
| Succinate | 5 mm |
| Sucrose | 220 mm |
| Prepare fresh from stock solutions before use. | |
- © 2014 Cold Spring Harbor Laboratory Press
