Na+-Containing Solution for Measuring Ca2+ Fluxes across the Mitochondrial Inner Membrane
| Reagent | Final concentration |
|---|---|
| Cyclosporin A | 1 µm |
| EGTA | 0.4 mm |
| HEPES–KOH (pH 7.4) | 10 mm |
| NaCl | 20 mm |
| KH2PO4 | 2.5 mm |
| Ruthenium red | 1 µm |
| Succinate | 5 mm |
| Sucrose | 220 mm |
| Prepare fresh from stock solutions before use. | |
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