Na+-Free Solution for Measuring Ca2+ Fluxes across the Mitochondrial Inner Membrane

Recipe

Na⁺-Free Solution for Measuring Ca²⁺ Fluxes across the Mitochondrial Inner Membrane

Reagent	Final concentration
Cyclosporin A	1 µm
EGTA	0.4 mm
HEPES–KOH (pH 7.4)	10 mm
KH₂PO₄	2.5 mm
N-methyl-d-glucamine	20 mm
Succinate	5 mm
Sucrose	220 mm

Prepare fresh from stock solutions before use.

doi:10.1101/pdb.rec076265 Cold Spring Harb Protoc 2014. 2014: pdb.rec076265- © 2014 Cold Spring Harbor Laboratory Press