RNAi Medium

• 1. Prepare 1 L of nematode growth medium (NGM) by following Steps 1–4 of the NGM recipe.

• 2. Add 2.5 mL of 0.1 m isopropylthio-β-galactoside (IPTG) and 500 µL of 50 mg/mL carbenicillin.

• 3. Using an automated plate pourer (peristaltic pump), pour the medium into sterile plastic plates to set. During pouring, keep the medium on a hot plate with stirrer to prevent the medium from solidifying.

• 4. Store RNAi plates for up to 1 mo at 4^oC.

• © 2014 Cold Spring Harbor Laboratory Press