Recipe

Internal Solution

Reagent Final concentration (mm)
KCl  12
Potassium gluconate 130
HEPES buffer  10
Mg-ATP  4
NaCl  4
Adjust the pH to 7.2 with KOH and the osmolarity to 290 mOsm. Recheck after adding the dye.
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  1. doi:10.1101/pdb.rec081430 Cold Spring Harb Protoc 2014: pdb.rec081430- © 2014 Cold Spring Harbor Laboratory Press
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