Hypotonic Buffer for Drosophila Cells
| Reagent | Quantity (for 250 mL) | Final concentration |
|---|---|---|
| HEPES–KOH (1 m, pH 7.6) | 0.5 mL | 10 mm |
| MgCl2 (1 m) | 375 µL | 1.5 mm |
| KCl (1 m) | 2.5 mL | 10 mm |
| Dithiothreitol (DTT; 0.5 m) | 250 µL | 0.5 mm |
| H2O | to 250 mL | |
| Add DTT immediately before use. Store at 4°C. The solution will keep for 6 mo to 1 yr. | ||
- © 2014 Cold Spring Harbor Laboratory Press
