Recipe

IPB1 Buffer

Reagent Quantity (for 100 mL) Final concentration
Tris–HCl (1 m, pH 7.5)    1 mL  10 mm
NaCl (5 m)    3 mL 150 mm
MgCl2 (1 m)  0.25 mL  2.5 mm
Triton X-100 (10%)   0.5 mL  0.05 %
Phenylmethylsulfonylfluoride (PMSF; Sigma-Aldrich P7626; 0.1 m in isopropanol)   0.1 mL  0.1 mm
Protease inhibitor cocktail (1000×)   0.1 mL     1×
Cold H2O to 100 mL
Store at 4°C. Add PMSF and protease inhibitor cocktail immediately before use.
No Related Web Pages
| Table of Contents

This Article

  1. doi:10.1101/pdb.rec081505 Cold Spring Harb Protoc 2014: pdb.rec081505- © 2014 Cold Spring Harbor Laboratory Press
  1. » Full Text

Article Category

  1. Recipe

Personal Folder

  1. Save to Personal Folders

Updates/Comments

  1. Submit Updates/Comments
  2. No Updates/Comments published
  3. Alert me when Updates/Comments are published

Related Content

  1. Related Web Pages

Share

Current Issue

  1. March 2026, 2026 (3)
  1. Current Issue