Homemade [5′-32P]pCp
| Reagent | Quantity |
|---|---|
| OHcP (cytidine-3′-monophosphate) (1 mm stock) | 1 µL |
| OptiKinase buffer | 4 µL |
| [γ-32P]ATP (150 µCi/μL; 6000 Ci/mm) | 33 µL |
| OptiKinase (Affymetrix) | 2 µL |
| Incubate for 1 h at 37°C. Treat for 10 min at 65°C and store at −20°C. | |
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