RNA Ligase Buffer (10×)
| Reagent | Quantity (for 1.0 mL) | Final concentration (10×) |
|---|---|---|
| Tris–HCl (1 m, pH 8.0) | 500 µL | 0.5 m |
| MgCl2 (1 m) | 150 µL | 0.15 m |
| Dithiothreitol (DTT) (0.5 m) | 66 µL | 33 mm |
| H2O | 284 µL | |
| Store for up to 6 mo at −20°C. | ||
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