T4 Kinase Buffer (10×)
| Reagent | Quantity (for 1 mL) | Final concentration (10×) |
|---|---|---|
| Tris-HCl (1 m, pH 7.5) | 500 µL | 0.5 m |
| MgCl2 (1 m) | 100 µL | 0.1 m |
| Dithiothreitol (DTT) (0.5 m) | 100 µL | 50 mm |
| H2O | 300 µL | |
| Store for up to 6 mo at −20°C. | ||
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