E2 Buffer for ChIP
| Reagent | Amount (for 50 mL) | Final concentration |
|---|---|---|
| Tris–HCl (1 m, pH 8.0) | 500 µL | 10 mm |
| NaCl (5 m) | 2 mL | 200 mm |
| EDTA (0.5 m, pH 8.0) | 100 µL | 1 mm |
| EGTA (0.5 m, pH 8.0) | 50 µL | 0.5 mm |
| Double-distilled water | 47.35 mL | – |
| Store for up to several months at 4°C. | ||
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