E3 Buffer for ChIP
| Reagent | Amount (for 50 mL) | Final concentration |
|---|---|---|
| Tris–HCl (1 m, pH 8.0) | 500 µL | 10 mm |
| NaCl (5 m) | 2 mL | 200 mm |
| EDTA (0.5 m, pH 8.0) | 100 µL | 1 mm |
| EGTA (0.5 m, pH 8.0) | 50 µL | 0.5 mm |
| Sodium deoxycholate (10% [w/v]) | 500 µL | 0.1% (w/v) |
| Sodium N-lauroylsarcosine (10% [w/v]) | 2.5 mL | 0.5% (w/v) |
| Double-distilled water | 44.35 mL | – |
| Store for up to several months at 4°C. | ||
- © 2014 Cold Spring Harbor Laboratory Press
