Recipe

HEG Solution

Reagent Amount for 10 mL Final concentration
HEPES–KOH (1 m, pH 7.5) 500 µL 50 mm
EDTA (0.5 m, pH 8.0)  20 µL  1 mm
Glycerol (100%)  2 mL  20%
Double-distilled water 7.5 mL   –
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  1. doi:10.1101/pdb.rec082719 Cold Spring Harb Protoc 2014: pdb.rec082719- © 2014 Cold Spring Harbor Laboratory Press
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