Recipe

Normal Amphibian Medium

Reagent Concentration (10×)
NaCl  1.1 m
KCl 20 mm
Ca(NO3)2 10 mm
MgSO4 10 mm
EDTA  1 mm
Use this solution at 0.1×, supplemented with 2 mm sodium phosphate (pH 7.5) from a 50× stock (0.1 m sodium phosphate buffer).
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  1. doi:10.1101/pdb.rec082768 Cold Spring Harb Protoc 2014: pdb.rec082768- © 2014 Cold Spring Harbor Laboratory Press
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