RIPA Buffer for Xenopus ChIP
| Reagent | Amount (for 50 mL) | Final concentration |
|---|---|---|
| HEPES–KOH (1 m, pH 7.5) | 2.5 mL | 50 mm |
| LiCl (7.5 m) | 3.33 mL | 500 mm |
| EDTA (0.5 m, pH 8.0) | 100 µL | 1 mm |
| Igepal CA-630 (10%) | 5 mL | 1% |
| Sodium deoxycholate (10% [w/v]) |
3.5 mL | 0.7% |
| Double-distilled water | 35.6 mL | – |
- © 2014 Cold Spring Harbor Laboratory Press
