Normal Amphibian Medium
| Reagent | Concentration (10×) |
|---|---|
| NaCl | 1.1 m |
| KCl | 20 mm |
| Ca(NO3)2 | 10 mm |
| MgSO4 | 10 mm |
| EDTA | 1 mm |
| Use this solution at 0.1×, supplemented with 2 mm sodium phosphate (pH 7.5) from a 50× stock (0.1 m sodium phosphate buffer). | |
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