Hematoxylin Solution (Mayer's)

• 1. Add 50.0 g of aluminum ammonium sulfate to 800 mL double-distilled H₂O. Stir, but do not use heat to assist dissolving.

• 2. Add 1.2 g of hematoxylin crystals and stir until dissolved.

• 3. Add 0.2 g of sodium iodate and 1.0 g of citric acid. Stir until dissolved.

• 4. Add 50 g of chloral hydrate and stir overnight at room temperature.

  • Chloral hydrate is a controlled substance; check with your laboratory safety officer regarding purchase and appropriate/safe storage.

• 5. Bring the total volume up to 1000 mL (this usually takes ∼100 mL H₂O). Filter and store in a dark bottle at room temperature.

  • Mayer's hematoxylin keeps well for several months. This recipe is based on one described by the Armed Forces Institute of Pathology (1968).

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