Recipe

ICRAC Ca2+-Free Bath Solution

NaCl 150 mm
Tetraethylammonium chloride (TEA-Cl)  10 mm
MgCl2  2 mm
Glucose  30 mm
HEPES  10 mm
Adjust pH to 7.2 with NaOH (not below 7.2 because CRAC channels are blocked by low pH, a pH of up to 7.4 can also be used). Adjust osmolarity to around 320 mOsm. Sterile-filter before storage.
| Table of Contents

This Article

  1. doi:10.1101/pdb.rec076323 Cold Spring Harb Protoc 2014: pdb.rec076323- © 2014 Cold Spring Harbor Laboratory Press
  1. » Full Text

Article Category

  1. Recipe

Personal Folder

  1. Save to Personal Folders

Updates/Comments

  1. Submit Updates/Comments
  2. No Updates/Comments published
  3. Alert me when Updates/Comments are published

Share

Current Issue

  1. April 2026, 2026 (4)
  1. Current Issue