ICRAC Pipette Solution for Passive Depletion
| Cesium aspartate | 120 mm |
| EGTA (or tetracesium BAPTA) | 10 mm |
| HEPES | 10 mm |
| MgCl2 | 3 mm |
| NaCl | 8 mm |
| Adjust pH to 7.2 with CsOH (not below 7.2 because CRAC channels are blocked by low pH, a pH of up to 7.4 can also be used). Adjust osmolarity to ∼300 mOsm. Sterile-filter, then aliquot and store at −20°C. Note that some buffering agents (e.g., BAPTA) are unstable at RT and one should follow instructions of the supplier for storage and handling. | |
- © 2014 Cold Spring Harbor Laboratory Press
