Poly(A) Polymerase Buffer (5×)
| Reagent | Quantity (for 1.0 mL) | Final concentration (5×) |
|---|---|---|
| Tris–HCl (1 m, pH 8.0) | 100 µL | 0.1 m |
| MnCl2 (0.3 m) | 10 µL | 3 mm |
| EDTA (0.1 m) | 1 µL | 0.1 mm |
| DTT (0.5 m) | 2 µL | 1 mm |
| Bovine serum albumin (BSA), acetylated (50 mg/mL) | 10 µL | 500 µg/mL |
| H2O | 877 µL | |
| Store the buffer indefinitely at –20°C. | ||
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