Recipe

HEPES-Buffered Hanks Balanced Salt Solution without Ca2+ and Mg2+ (Ca2+/Mg2+-free HHBSS)

Reagent Final concentration
HBSS (10×, without Ca2+, Mg2+, or phenol red; Life Technologies)   1×
HEPES  20 mm
d-Glucose   2 g/L
Prepare the solution in Chelex-treated ultrapure H2O. Adjust pH to 7.2 and sterilize using a bottle-top 0.22-μm filter. Store solution in a metal-free plastic container.

| Table of Contents

This Article

  1. doi:10.1101/pdb.rec077271 Cold Spring Harb Protoc 2015: pdb.rec077271- © 2015 Cold Spring Harbor Laboratory Press
  1. » Full Text

Article Category

  1. Recipe

Personal Folder

  1. Save to Personal Folders

Updates/Comments

  1. Submit Updates/Comments
  2. No Updates/Comments published
  3. Alert me when Updates/Comments are published

Share

Current Issue

  1. March 2026, 2026 (3)
  1. Current Issue