Caspase Reaction Buffer
| Reagent | Final concentration |
|---|---|
| HEPES | 100 mm |
| Glycerol | 20% |
| EDTA | 0.5 mm |
| CHAPS | 0.1% |
| Pefabloc | 0.5 mm |
| Dithiothreitol (DTT) | 8 mm |
| Ac-DEVD–7-amino-trifluoromethylcoumarin (AFC) substrate (Enzo Life Sciences) | 50 µm |
| Prepare a stock solution that contains the first four ingredients, adjust pH to 7.5, and store it at 4°C. Immediately before use, add the last three ingredients. | |
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