Digitonin Extraction Buffer
| Reagent | Final concentration |
|---|---|
| Sucrose | 250 mm |
| HEPES | 20 mm |
| KCl | 10 mm |
| MgCl2 | 1.5 mm |
| EDTA | 1 mm |
| EGTA | 1 mm |
| Pefablock | 0.5 mm |
| Prepare a stock solution that contains the first six ingredients, adjust pH to 7.5, and store it at 4°C. Immediately before use, add the Pefablock. | |
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